Search results for " cell lysis"
Article
Challenges in Cell Harvesting Prompt Enhancements
This minimizes the shear forces on the liquids and prevents cell lysis. The feed zone is completely filled with rotating liquid, creating an air-tight environment. Because the system is completely her…
Article
Single-Use Technologies Prove Effective for Viral Vector Process Development
The presence of serum, culture harvest viability, turbidity, cell lysis reagents, contaminant DNA levels, and residual transfection agents are all upstream factors. Overall process scalability and rob…
Article
Gene Therapies Push Viral Vector Production
Gene Therapies Push Viral Vector Production
Viral vectors show promise as a delivery mechanism for gene therapy, but which virus types are commercially viable?
By Feliza Miraso…
Article
Host-Cell Protein Measurement and Control
Jun 01, 2015
By Fengqiang Wang, PhD, Daisy Richardson, Mohammed Shameem
BioPharm International
Volume 28, Issue 6, pg 32–38
Host-cell proteins (HCPs) constitute a major part of proce…
Article
Real-Time Characterization of Biotherapeutics and Comparability of Biosimilars
Real-Time Characterization of Biotherapeutics and Comparability of Biosimilars
A major advantage of SPR-based analysis is its ability to estimate the association and dissociation rate constants, an …
Article
Top Process Development Trends for 2021 and a Look into 2022
Article
Challenges of Protein Aggregation During Purification
“Shear can cause protein aggregation directly or indirectly via cell lysis, which leads to aggregation catalyzed by enzymatic reactions,” explains Vedantham. He adds that exposure of proteins at the a…
Article
Continuous Processing for the Production of Biopharmaceuticals
…opment of other separation technologies such as continuous chromatography, refolding, precipitation, cell lysis, filtration, and aqueous two-phase extraction (3). Table I presents a summary of recent…
Article
Top Challenges to Overcome When Developing AAV Processes: Part 1 of 2
…transfection
4:27 – Three main challenges for downstream AAV process development, including cell lysis and the polishing step for full capsid enrichment
7:28 – Challenges associated wit…
Article
Optimization of Adenovirus Midstream Purification Steps
Adenovirus (AdV)-based vectors are widely evaluated as vaccine delivery system in preclinical and clinical studies for various infectious diseases. In this application note, studies of midstream pur…